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Fig. 2 | BMC Energy

Fig. 2

From: Recent advances in high-throughput mass spectrometry that accelerates enzyme engineering for biofuel research

Fig. 2

Colorimetric and MS analyses of cellulose-degrading reactions. a Schematic illustration on enzymatic deconstruction of celluloses into oligosaccharide products. CHO indicates the reducing ends of glycans. b Colorimetric measurement of reducing sugar equivalents using the 3,5-dinitrosalicylic acid (DNS) reagent. The resultant 3-amino-5-nitrosalicylic acid exhibits a specific absorption at 540 nm. c A schematic MALDI mass spectrum of cellulolytic degradation products to reflect product specificity of a select cellulase by detecting and quantifying various released oligosaccharides, which produce different peaks of corresponding m/z values in a mass spectrum

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