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Fig. 1 | BMC Energy

Fig. 1

From: Recent advances in high-throughput mass spectrometry that accelerates enzyme engineering for biofuel research

Fig. 1

High-throughput MS approaches to screen enzyme libraries. a MALDI MS imaging-based screening. Enzyme reactions are spotted as microarrays on a surface called MALDI target. Then, matrix molecules that absorb laser energy are overlaid. Laser shots are fired at high frequencies across surface microarrays for analyte desorption, ionization, and MS analysis. b Microfluidic loading to ESI MS. Enzyme reactions are performed in aqueous droplets in microfluidic channels followed by direct infusion into an ESI source. Adapted with Permission from [24]. Copyright 2018 American Chemical Society. c Acoustic loading to ESI MS. Enzyme reactions are created in microtiter plates. From each well, nanoliter droplets are ejected using an acoustic transducer. The droplets are then introduced to an ESI source via an open port probe (OPP) that carries a continuous solvent flow

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